Application of soil microorganism to induce plant resistance against Tobacco mosaic virus
Date Issued
2014
Date
2014
Author(s)
Wang, Po-Chuan
Abstract
Recent research indicated that the secondary metabolites of soil microorganisms can induce plant resistance against fungus and bacterial disease; however, whether the metabolites are effective to virus disease remain to be resolved. Thus, we selected Nicotiana glutinosa, a local lesion host of Tobacco mosaic virus (TMV), as the indicator plant for analysis. The local lesion numbers induced by TMV were counted on plants treated with culture filtrates derived from soil microorganisms to measure the resistance response. The culture filtrates of the microorganisms was sprayed on to adaxial surface of plants then inoculated with TMV on the adaxial leaf surface to screen the filtrate with potential to induce the antiviral activity. Filtrate derived from two microorganisms can reduced 90% lesions on treated N. glutinosa. When the culture filtrates was sprayed on the leaves abaxial surface and inoculated the TMV on the adaxial surface of plants. About 60% - 65% lesions reduction was observed. It indicated that these culture filtrates can induce systemic protection against virus infection. Besides the TMV susceptible cultivar Nicotiana benthamiana was also selected for analysis, and the disease index was set up for further analysis. The culture filtrates was sprayed on lower leaves and then inoculated the TMV on the upper leaves, the symptoms delay was observed. It indicate that the culture filtrates of these two fungi have the ability to induce plant systemic protection. The hydrogen superoxide (H2O2) accumulation and pathogen-related protein gene, PR-1a, expression were detected on N. benthamiana leaves pretreated with the culture filtrates. These two fungi were identified by 26S rDNA D1/D2 region, ITS sequence analysis and morphology, they are highly related to Trichosporon scarabaeorum and Scedosporium dehogii. Our studies suggested that most of filtrates derived from soil microorganisms can induce plant resistance against viruses. We isolated two fungi from soil, and the filtrates derived from the two fungi can induce prominent salicylic acid related plant resistance response both on susceptible and resistance plants, and the potential application of the isolated fungi on virus disease management is discussed.
Subjects
土壤微生物
菸草嵌紋病毒
誘導抗性
次級代謝物
Type
thesis
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