Cloning and analysis of flagellin genes from Salmonella enterica serovar Choleraesuis isolated from Taiwan
Date Issued
2007
Date
2007
Author(s)
Wang, Fang-Wei
DOI
zh-TW
Abstract
Salmonella enterica serovar Choleraesuis (S. Choleraesuis) belonged to the family Enterobacteriaceae and was a host-adapted, facultative, intracellular pathogen. It could cause the diseases in human and pigs, but more serious in pigs. It was a gram-negative bacterium with peritrichous flagella. Flagellum, a hair-like appendage, was the bacterial locomotive structure. A flagellum could be subdivided into three parts: basal body, hook and filament which composed of flagellin protein. Flagella had also been recognized as one of the potential virulence factors of microorganisms. Diphasic Salmonella enterica strains possessed two nonallelic structural genes, fliC and fljB, encoding phase I and II flagellin, respectively, with different antigenecity. A phenomenon called phase variation that a bacterium expressed alternately phase I and II flagellin. Our lab had cloned and characterized the phase I fliC gene from S. Choleraesuis 12440 previously. There was an insert of 36 nucleotides absent in other phase I flagellin genes. In this study, phase II flagellin gene fljB was cloned and characterized from S. Choleraesuis 12440. One hundred and ninety-nine strains of S. Choleraesuis collected from Taiwan by Taizhong center for disease control (CDC) were analyzed using PCR technique. The data indicated all the fliC genes of 199 strains had the same structure as that of strain 12440 with a 36-bp insert. One hundred and ninety-eight strains and strain CH12440 had a fljB gene with a IS10 (insertion sequence 10). The fljB without transposon insert was cloned and expressed. The toxicity of expressed FljB protein on mouse macrophage cell lines was studied by MTT (methythiazoletetrazolium) assay. FljB protein displayed, as FliC , a cytotoxic effect on the cells.
Subjects
豬霍亂沙門氏桿菌
鞭毛
相位變異
細胞毒殺能力
S. Choleraesuis
Flagellum
phase variation
cytotoxic effect
SDGs
Type
other
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