Investigation of the wound healing and anti-asthma effects of velvet antler of Formosa sambar deer in vitro and in vivo
Date Issued
2012
Date
2012
Author(s)
Wang, Ting
Abstract
Velvet antler (VA) is one of the most famous and valuable Chinese traditional medicines. Several studies indicated that the VA comprises certain growth factors related to its regeneration annually. These growth factors might provide positive effects on wound repairs. In addition, in our previous study, we also demonstrated the anti-allergic and anti-inflammatory effects of VA from Formosa sambar deer (Rusa unicolor swinhoii). Thus, the purpose of this study was to further evaluate the effects of VA and its extracts (VA cold-water-soaked and VA water-boiled extracts) from Formosan sambar deer on the wound healing and anti-asthmatic effects in vitro and in vivo.
For wound healing study, the human keratinocyte cells (HaCaT) were co-incubated with VA extracts. Results indicated that both VA cold-water-soaked (VACWS) and VA water-boiled-extract (VAWB) samples could stimulate keratinocyte proliferation in a dosage-dependent manner. VACWS were also demonstrated a significantly higher wound repairing ability than other samples in a dosage-dependent manner using cell migration assay. On the other hand, the VA extracts were co-incubated with human fibroblast cells (CCD-966SK). The results indicated that both VA cold-water-soaked (VACWS) and VA water-boiled-extract (VAWB) samples have no significant effect on cell proliferation. The wound healing animal model did not show any significant difference among treatments in wound healing. For anti-asthmatic study, the asthmatic mice induced by ovalbumin were used to evaluate the anti-hyper-responsive effect of the VA powder. The results clearly demonstrated that the VA powder has an anti-asthma effect with ameliorating the symptom of airway hyper-responsiveness (AHR). The levels of Type 2 helper T cell (Th2) cytokines, such as interleukin (IL)-4, IL-5 and IL-13, in brochoalveolar lavage (BAL) were significantly decreased. The total eosinophil count induced by ovalbumin in BAL was also reduced. Interestingly, OVA-specific IgE in mice serum was not reduced by the VA treatments.
In summary, these findings clearly revealed that VACWS has potential for wound healing and the VA powder could ameliorate the symptom of AHR. Further studies are necessary to reveal the mechanisms and to determine the possible compounds involved in the wound repairs and anti-allergic effects.
Subjects
Velvet antler
Formosan sambar deer
Cell proliferation
Cell migration
Wound healing
Asthma
Airway hyper-responsiveness
SDGs
Type
thesis
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