A Step-by-Step Optimization Guide for Applying Tissue Specific RNA in-situ Hybridization to Non-Model Plant Species
Resource
Taiwania, 53(4), 383-393
Journal
Taiwania
Journal Volume
53
Journal Issue
4
Pages
383-393
Date Issued
2008-12
Date
2008-12
Author(s)
Abstract
Tissue specific RNA in-situ hybridization is a critical technique required for botanists to examine exact gene expression domain within plant tissues. This powerful technique provides a way to examine the spatial and temporal patterns of gene expression of plants and animals in developmental stages content and at tissue cellular level. However, this technique has been largely hampered by difficulties of obtaining reliable protocols for non-model organism. Here we offer our lab experiences on how to optimize in-situ hybridization process among various plant species. This step-by-step guide however should not be regarded as the only solution to resolve the difficulties of RNA tissue specific RNA in-situ hybridization. Instead, the key optimization steps including tissue fixation time, proteinase/pronase treatment, probe length/content and hybridization time are priority issues to be tested first. We further summarized some recent advance of literature on alternative ways to conduct in-situ hybridization process.
Subjects
digoxigenin (DIG) – labeled RNA probes
histone gene
in-situ hybridization
African violet
Titanotrichum oldhamii
毛地黃素非放射性雜交探針
組織蛋白基因
原位雜交技術
非洲堇
俄氏草
SDGs
Type
journal article
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