Proteinase Inhibitor II Gene Transfer to Rice Plants for Insect Resistance
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
劉麗飛
DOI
912313B002322
Abstract
pinII) gene, from potato tuber, were
transferred to rice plant for increasing the
insect resistant ability of rice plant. PinII
gene was driven by its original PinII
promoter, which causes gene expression only
when plant is attacked by insects, therefore
will not interfere the plant normal growth.
The constructed gene was transferred to rice
TNG67 and TK9 by Agrobacterium method.
At present transformation has been done
four times. Seven independent T0 lines were
obtained from TNG67. More T0 lines will be
obtained soon. PI activities of each T0 plants
were detected by BAPNA method and
showed 3.5 ~ 9 folds higher than that of
non-transgenic plants.
Subjects
Rice
Gene transfer
Proteinase
inhibitor (PI)
inhibitor (PI)
insect resistant
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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