A Polymeric Cell Counter with integrated Micro-pipette as Aperture
Date Issued
2004
Date
2004
Author(s)
Lai, Yen-Hsun
DOI
en-US
Abstract
The thesis designs and fabricates a micro-coulter counter in sensing voltage pulse when a cell traveling through the aperture. And from signal, cell number and size information can be obtained. Two ways of fabrication are presented. First, using soft-lithography and pulled pipette, we integrate polydimethylsiloxane (PDMS) channel with only the tip section of a micro-pipette to perform cell/particle
counting. Second, the whole pipette (tip and constant diameter regions) is inserted into a PDMS channel.
The advantage of this integration is that one can use inexpensive PDMS to fabricate the overall device while the tip of pulled pipette, with a specific diameter, can be used as the aperture for a particular cell/particle population.
By four-probe measurement, voltage potential differences will be get via pre-amplifier and filter. Using LabView® for data acquisition, we measured the changes of resistance to determine the count and the size of the particle.
Experiments include the calibration of the electric signal, poly-spheres counting, red/white blood cell counting, and signal amplitude with different aperture size. Consequences show that the detected signals and waveforms of digital signal process. By the calculation, distribution of different mean diameter particles is shown with the histogram.
Advantage of our count chip is that it can easily change the aperture diameter preparation via a molding process using common apparatus. The results provide the reference of cell counting and diameter distribution. And easy
fabrication makes it convenient in integration with micro-fluidic chip.
Results show that counting of red and white blood cells are successfully achieved. Proper grounding and signal conditioning are very critical in obtaining quality results.
counting. Second, the whole pipette (tip and constant diameter regions) is inserted into a PDMS channel.
The advantage of this integration is that one can use inexpensive PDMS to fabricate the overall device while the tip of pulled pipette, with a specific diameter, can be used as the aperture for a particular cell/particle population.
By four-probe measurement, voltage potential differences will be get via pre-amplifier and filter. Using LabView® for data acquisition, we measured the changes of resistance to determine the count and the size of the particle.
Experiments include the calibration of the electric signal, poly-spheres counting, red/white blood cell counting, and signal amplitude with different aperture size. Consequences show that the detected signals and waveforms of digital signal process. By the calculation, distribution of different mean diameter particles is shown with the histogram.
Advantage of our count chip is that it can easily change the aperture diameter preparation via a molding process using common apparatus. The results provide the reference of cell counting and diameter distribution. And easy
fabrication makes it convenient in integration with micro-fluidic chip.
Results show that counting of red and white blood cells are successfully achieved. Proper grounding and signal conditioning are very critical in obtaining quality results.
Subjects
細胞計數
電阻抗
PDMS
coulter counter
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-93-R90543005-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):6856af6d8007af0a3990d6b155deb765