MicroRNA-like small RNAs prediction in the development of antrodia cinnamomea
Journal
PLoS ONE
Journal Volume
10
Journal Issue
4
Date Issued
2015
Author(s)
Lin Y.-L.
Ma L.-T.
Lee Y.-R.
Lin S.-S.
Wang S.-Y.
Chang T.-T.
Shaw J.-F.
Li W.-H.
Abstract
Antrodia cinnamomea, a precious, host-specific brown-rot fungus that has been used as a folk medicine in Taiwan for centuries is known to have diverse bioactive compounds with potent pharmaceutical activity. In this study, different fermentation states of A. cinnamomea (wild-type fruiting bodies and liquid cultured mycelium) were sequenced using the next-generation sequencing (NGS) technique. A 45.58 Mb genome encoding 6,522 predicted genes was obtained. High quality reads were assembled into a total of 13,109 unigenes. Using a previously constructed pipeline to search for microRNAs (miRNAs), we then identified 4 predicted conserved miRNA and 63 novel predicted miRNA-like small RNA (milRNA) candidates. Target prediction revealed several interesting proteins involved in tri-terpenoid synthesis, mating type recognition, chemical or physical sensory protein and transporters predicted to be regulated by the miRNAs and milRNAs.
Type
journal article