Light sheet fluorescence microscopy in NIR II for mouse lung imaging
Journal
Proceedings of SPIE - The International Society for Optical Engineering
Journal Volume
12608
ISBN
9781510663435
Date Issued
2023-01-01
Author(s)
Abstract
Light sheet fluorescence microscopy (LSFM) is one of the leading technique for high resolution imaging. To image mouse lungs, optical sectioning and high-speed image acquisition are required. In addition, light scattering, reflection, and attenuation limit the application of light sheet microscopy in the visible region to imaging small and transparent animals and organisms. In this work, we present a light sheet microscope that works in the NIR-II wavelength range and demonstrate deep mouse lung imaging. We utilized light with excitation at 532 nm and detection in the NIR-II range of approximately 1450 nm. Our method is expected to find important applications for deep tissue imaging.
Subjects
light sheet microscopy | near infrared | Optical-sectioning microscopy
Type
conference paper