QTL Mapping for Chilling Tolerance at Seedling Stage in Rice (Oryza sativa L.)
Date Issued
2012
Date
2012
Author(s)
Yang, Pei-Wen
Abstract
Rice grown in temperate areas is subjected to chilling stress at seedling and booting stages. The purpose of this thesis was to identify quantitative trait loci (QTLs) for chilling tolerance at the seedling stage of rice, using 243 recombinant inbred lines derived from a cross between a chilling-tolerant japonica cultivar, TNG67, and a chilling-sensitive indica cultivar, TN1. The phenotypes of chilling tolreance at seedling stage was scored by the measurements of the survival rate and the artifical chilling-tolerant classes. The results showed that the correlation coefficient between the survival rate and the artifical chilling-tolerant classes was 0.50. Both phenotypic measurements identified two major QTLs on the same chromosomal region: one on chromosome 6, the other on the chromosome 11. The former QTL linked to the SNP marker RC6-1, and the latter QTL linked to the SNP marker RC11-11. The QTLs for the survival rate under chilling stress were named qCTSS6.1 and qCTSS11.1, and the QTLs for the chilling-tolerant classes were named qCTS6.1 and qCTS11.1 The combined effects of the two QTLs were able to explain approximately 30% of phenotypic varions within the genetic mapping population, for either the survival rate or the chilling-tolerant classes.
Subjects
QTL
Chilling Tolerance
Rice
TNG67
TN1
Type
thesis
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