The further characterization of the specifically binding peptide to hepatocellular carcinoma
Journal
Biomedical Engineering - Applications, Basis and Communications
Journal Volume
26
Journal Issue
6
Date Issued
2014
Author(s)
Abstract
Previously, we reported a novel 12-mer peptide binding to hepatocellular carcinoma (HCC) cells specifically and sensitively, and it was screened by using a phage displayed peptide library. To identify the potential of this peptide to be used to the early diagnosis and drug delivery of HCC clinic, the synthesized peptide probe was further characterized by using the assays of cell immunofluorescence and tissue chip. The results of cultured cell immunofluorescence assay show that the peptide probe binds to HCC cells with satisfactory specificity and sensitivity. The results of tissue chip show that the peptide probe can bind to the most common types of HCC tissues, especially to the HCC tissues differentiated at grade II. All further characterized results indicate that this peptide is of the great potential to be used as a HCC targeting candidate for the early molecular imaging diagnosis and targeting nanoparticle or drug delivery in HCC clinic practice. ? 2014 National Taiwan University.
Subjects
Hepatocellular carcinoma; Specific peptide; Targeting diagnosis and therapy
SDGs
Other Subjects
Assays; Bins; Diagnosis; Fluorescence; Histology; Molecular imaging; Probes; Tissue; Binding peptide; Cultured cell; Early diagnosis; Hepatocellular carcinoma; Immunofluorescence assay; Peptide binding; Phage displayed peptide; Targeting diagnosis and therapy; Peptides; HCSP4 peptide; peptide; unclassified drug; Article; cancer diagnosis; controlled study; drug delivery system; early diagnosis; fluorescence imaging; human; human cell; human tissue; liver cell carcinoma; molecular imaging; molecular probe; parasitology immunofluorescence assay; protein binding; protein localization; protein synthesis; sensitivity and specificity
Type
journal article