Crowding alters the dynamics and the length of RecA nucleoprotein filaments in RecA-mediated strand exchange
Journal
ChemPhysChem
Journal Volume
15
Journal Issue
1
Pages
80-84
Date Issued
2014
Author(s)
Wu, H.-Y.
Abstract
Crowd impact: Molecular crowding effects of bovine serum albumin and poly(ethylene glycol) on the Escherichia coli RecA-mediated strand exchange reaction are quantified by using a single-molecule outgoing strand experiment and magnetic pull-down and ATPase assays. The alterations of the biochemical parameters of this complex enzymatic reaction in such crowded environments are discussed.
Type
journal article