使用限制酶位點標定之核酸定序法進行稉稻雜交組合之穗上發芽數量性狀基因座的遺傳定位

Preharvest sprouting refers to ripe kernels germinate before harvest, which leads to yield loss and poor grain quality. A rice F2 genetic mapping population was created by the cross of TK14, a variety with high preharvest sprouting
rate, and TY1, a variety with low preharvest sprouting rate, and then conducted QTL analysis for preharvest sprouting and seed dormancy. A total of 60 individuals with highest and lowest preharvest sprouting rate in the F2 population were selected, and their DNA were extracted to do RAD (restriction-site associated DNA) sequencing, in order to develop sufficient number
of SNP markers as well as to identify these SNP marker genotypes of the selected individuals simultaneously. F2:3 families from the selected F2
individuals were also generated and the relationship between preharvest sprouting and seed dormancy was investigated. The results showed that QTLs for both preharvest sprouting and seed dormancy were detected on the same
region of chromosome 3.