In Vivo Whole-Cell Recording in the Gerbil Cochlear Nucleus
Journal
Neuromethods
Journal Volume
176
Pages
305-320
ISBN
978-1-0716-2021-2
978-1-0716-2022-9
Date Issued
2022-01-01
Author(s)
Joris, Philip X.
Abstract
This chapter describes how to use patch-clamp electrodes to perform blind in vivo whole-cell recording in the cochlear nucleus of anesthetized gerbils. This technique offers low-noise and stable recording of the intracellular membrane responses to sound stimuli, which is important for understanding the mechanisms underlying diverse sound-encoding schemes in the nucleus. With biocytin inside the pipette, it is also possible to label the recorded cell and link its anatomical features with physiology. Although in vivo whole-cell recording in rodents has been developed for more than a decade and has been abundantly used in cortical areas, it has seen little use in the auditory brainstem. This chapter discusses the techniques and challenges specific to recording in the gerbil cochlear nucleus.
Subjects
Cochlear nucleus | In vivo recording | Sharp electrode | Single-cell labeling | Single-cell recording | Whole-cell patch clamp
Publisher
Humana Press Inc.
Type
book part