Study on gibberellin-regulated mechanism of stem elongation and gene transformation technology for and high yield and semi-dwarf varieties of Jatropha curcas.
Date Issued
2012
Date
2012
Author(s)
Tsai, Chi-Yu
Abstract
Jatropha curcas L. is the favorable specie as alternative biofuel resource owing to the approximately 40% of oil in the seed. However, it harbors several disadvantageous characters, for example, less yield of net seeds and more than 2 meters of plant height causing inconvenient harvests and management. It is tempting to dissect functional role of gibberellins (GAs) on the growth height of J. curcas. The treatment of paclobutrazol (PAC), an inhibitor of GAs biosynthesis, brought about the dwarfism in J. curcas, indicating GAs inhibition is an available approach to obtain semi-dwarf plant. The spatial expression profile of GA-related genes revealed that GIBBERELLIN 20-OXIDASE 2 (JcGA20ox2) and GIBBERELLIN 2-OXIDASE 2 (JcGA2ox2) were two major genes playing roles in controlling plant height in J. curcas. Both of them also participated in GA-related feedback-regulatory mechanism. In this study, we established effective J. curcas regeneration and transformation system. Therefore, molecular breeding were carried out by transformation of Agrobacteriuim tumefaciens harbouring JcGA20ox2-RNAi and 35S::JcGA2ox2 in J. curcas. The results of this study would be potential to obtain high yield and semi-dwarf cultivars.
Subjects
biodiesel
gibberellin
Jatropha curcas
paclobutrazol
regeneration system
semi-dwarfism
transformation system
Type
thesis
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