Microencapsulation with porous starch carriers affects the storage stability and susceptibility of Bifidobacterium longum B6 to simulated gastric acid and bile salt
Date Issued
2010
Date
2010
Author(s)
Kao, Shu-Hui
Abstract
Microencapsulation is a method to protect dry probiotic powders. To understand if the survival rate of microencapsulated probiotic could be raised with the porous starch granules are taken as the carriers. In this study, using 10% porous starch obtained at different hydrolysis rate (30, 45, and 60%) and 10% gum arabic as the carriers, the microencapsulated cells of Bifidobacterium longum B6 were first prepared by spray-drying or freeze-drying. Survival of these microencapsulated and free cells of test organism in simulated gastric juice (pH 2.0) and bile solution (2%) as well as their stability during storage were examined.
In addition to the treatment group of native starch, we firstly hydrolyzed the corn starch granules with amyloglucosidase into three porous starch treatment groups: the one with hydrolysis rate of about 30, 45, and 60%. It was found that the porous starch with hydrolysis rate 30, 45, and 60% （porous starch-30, 45 and 60%） could be derived from native starch degraded for 1.5, 2.5, and 4 h, respectively. Survival of B. longum B6 is generally higher after freeze-drying than spray-drying. Freeze-drying with porous starch-30% + gum arabic or porous starch-45% + gum arabic. B. longum B6 showed a survival rate of 44.31 and 35.66%, respectively. While spray-drying with an outlet-air temperature of 50℃, B. longum B6 exhibited a survival rate of 33.85 and 14.95%, respectively. Elevation of outlet-air temperature resulted in reduced survival of treat organism. Regardless of drying method, B. longum B6 encapsulated with gum arabic alone or gum arabic + porous starch-30 or 45% showed a higher survival in the simulated gastric juice (pH 2.0) than the nonencapsulated cells. Encapsulated with porous starch-30 or 45% + gum arabic also exhibited a protective effect on test organism in 2% bile solution. An enhanced storage stability were also noted with these microencapsulated B. longum B6. Besides, survival of the test organism encapsulated with freeze-drying and held at 4℃ is higher than that encapsulated with spray-drying and stored at 25℃. Increasing porous starch carriers could slightly delay the decrease of the survival rate of bifidobacteria in the storage process. It revealed that using S30 or S45 as the carriers to microencapsulated probiotic could enhance the survival rate of probiotic in the processing.
Subjects
porous starch
Bifidobacterium
microencapsulation
drying
survival
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-99-R96641013-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):3f05e9fb6e0310fca88988d80018ce8c