Immunomodulation of the Active Compounds from Black Soybean [Glycine max (L.) Merr.] on Human Mononuclear Cells and the Antiproliferative Effects on U937 Cells
Date Issued
2006
Date
2006
Author(s)
Kung, Hao-Hsuan
DOI
zh-TW
Abstract
Black soybean [Glycine max (L.) Merr.] has been used as medicinal herb and food for hundreds of years. It also has been reported that black soybean contained numerous bioactive effect like radical-scavenging activity, anti-tumor activity, and activity for improving the fluidity of the whole blood. The water soluble portion of black soybean was precipitated by 75% alcohol and separated by Sephadex G-15 gel to obtain fraction 1(G15 P1) and fraction 2(G15 P2). These two fractions were used separately to stimulate human blood mononuclear cells (MNC) for one day to prepare conditioned medium (MNC-CM-1). U937 cells were incubated with condition medium for 5 days and the growth inhibition were studied. Results showed that G15 P2 stimulates human blood mononuclear cells to inhibit the cell growth of human myelocytic lymphoma U937 cells. It was also found that the MNC proliferated about 23.6 % and the MNC-CM-1 contained high levels of TNF-α, IL-1β and IFN-γ. However, in the investigation of the expression of monocyte-associated cell surface marker CD11b and CD14 on U937, we did not found differentiation of U937 cells after G15 P2 treatment. In the directly inhibitory experiments, we found G15 P1 at a dosage of 200
Subjects
黑豆免疫活性物質
免疫調節作用
U937細胞
生長抑制
Active Compounds from Black Soybean
Immunomodulation
U937 Cells
Antiproliferative Effects
Type
thesis
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