Antioxidant and antihepatoma activities of palm oil extract
Journal
Journal of Food Lipids
Journal Volume
14
Journal Volume
14
Journal Issue
2
Journal Issue
2
Pages
122-137
Start Page
122
End Page
137
ISSN
17454522
Date Issued
2007-06
Author(s)
Wu, Shu-Jing
Abstract
Palm oil extract (POE) at different concentrations (0.1-500 μg/mL) was evaluated for antioxidant and antihepatoma activities. Results showed that POE exhibited a greater antioxidant activity than α-tocopherol in all model systems tested. At concentrations of 0.1-100 μg/mL, POE showed a superoxide radical scavenging activity ranging from 50.6 to 100%, with an IC50 value (0.09 μg/mL) lower than α-tocopherol (0.51 μg/mL). In the xanthine oxidase inhibition test, POE (IC50 = 0.67 μg/mL) also exhibited a stronger inhibitory effect on xanthine oxidase activity than α-tocopherol (IC50 = 0.83 μg/mL). At concentrations of 0.1 μg/mL and above, POE showed significant ability in inhibiting the oxidative damage to lipids induced by ascorbate-Fe2+ than α-tocopherol in rat liver (IC50 = 0.53 μg/mL versus 0.94 μg/mL), brain (IC50 = 0.10 μg/mL versus 52.52 μg/mL) and plasma (IC50 = 77.17 μg/mL versus 190.81 μg/mL). In the antihepatoma studies, POE showed a greater potency than α-tocopherol in inhibiting Hep G2, Hep 3B and PLC/PRF/5 cells. The antiproliferative effect of POE against these human hepatoma cells was dose-dependent, with IC50 values varying from 4.86 to 68.44 μg/mL. The present results demonstrate that POE is an effective natural antioxidant supplement for protecting cellular membranes against oxidative damage and inhibiting hepatoma cell proliferation.
Subjects
Rattus
Type
journal article