Expression of Koi Herpesvirus in Peripheral Blood Leucocyte Cells Isolated from Latently Infected Koi
Date Issued
2011
Date
2011
Author(s)
Hsieh, Pei-Shan
Abstract
Koi herpesvirus (KHV), designated Cyprinid herpesvirus 3 (CyHV-3), is the etiological agent of an emerging and mortal disease in koi (Cyprinus carpio koi) and common carp (Cyprinus carpio carpio). The genome comprises a linear double-stranded DNA of 295 kbp. The disease is seasonal; afflict fish when the water temperature is 18 to 28°C. Virus propagation and virus gene transcription are turned off when cell are moved to a nonpermissive temperature of 30°C. And CyHV-3 can persist asymptomatically for long periods in the fish if the temperature out of optimal temperature or latency sustained, bursts of new infection occur after exposure to permissive temperatures and stress. Our previous data showed that thymidine kinase gene of KHV was detected in macrophages of infected koi by in situ hybridization and Weng found out that KHV TK gene copy number increased in the head kidney leukocyte derived from koi when detected by real-time PCR.
In our study, experimental result showed out, the mRNA of Orf4 (early transcription gene) and Orf2 (late transcription gene) can be detected both in KHV infected naive and carrier carp at 7-13 days postinfection (d.p.i.) when temperature at 23°C. But at 32°C only Orf4 could be detected, demonstrated the virus is unable to complete the replication in peripheral blood leucocytes (PBL) under nonpermissive temperature. In naive carp immersed with KHV the peak of the mortality at 10 d.pi. and serum neutralization (SN) titer can’t be detected within this duration at 23°C. Howerer at 32°C, the naive carp immersed with KHV showed normal appearance without death. The carrier carp exposed to KHV in both permissive and nonpermissive temperature appeared no death and higher SN titer. Moreover only Orf4 could be detected in TO and KF-1 that appeared not susceptibile to KHV, and both Orf4 and Orf2 can be detected in KHV infected TKF.
Subjects
KHV
CyHV-3
serum neutralization titer
carrier
Type
thesis
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