Effects of objective numerical apertures on achievable imaging depths in multiphoton microscopy
Journal
Microscopy Research and Technique
Journal Volume
65
Journal Issue
6
Pages
308-314
Date Issued
2004
Author(s)
Abstract
Multiphoton microscopy is a powerful technique for achieving three-dimensional submicron imaging in biological specimens. However, specimen optical parameters such as refractive indices and scattering coefficients can result in the loss of image resolution and decreased signal in depth. These factors are coupled to the focusing objective's numerical aperture (NA) in limiting the achievable imaging depths. In this work, we performed multiphoton imaging on aqueous fluorescent solution, human skin, and rat tail tendon to show that, under the same immersion condition, lower NA objectives can examine more deeply into biological specimens and should be used when optimal imaging depths is desired.
Type
journal article