栽培環境對稻米品質影響之蛋白質體研究：溫度調節性蛋白 質之功能分析(1/3)
Date Issued
2005-07-31
Date
2005-07-31
Author(s)
DOI
932313B002067
Abstract
Proteins are essential to rice caryopsis development and quality formation. High
temperature is an important environmental factor which may decrease grain quality.
In the present study rice caryopsis proteins were profiled by two-dimensional
polyacrylamide gel electrophoresis, and differentially expressed proteins were
analyzed by liquid chromatography/tandem mass spectrometry (LC\MS\MS).
Expressions of more than 400 polypeptide spots during caryopsis development, in
response to temperature treatments or between varieties were monitored. Among them,
more than 70 differentially expressed polypeptides were analyzed by LC\MS\MS. We
identified 54 proteins with known functions. Of these, 21 were involved with
carbohydrate metabolism, 14 with protein synthesis and sorting, and 9 with stress
responses. Waxy (Wx) proteins and glutelins were the most significant spots
increased significantly during development. Allergen-like proteins, PPDK and
NADH-SDH, also were expressed during development, implying their physiological
roles in caryopsis. Expression of large isoforms of Wx proteins was correlated with
the amylose content of rice caryopses. One protein with high GC content in its DNA
sequence was correlated with the chalky trait of kernels. High temperature (35/30 ℃)
decreased expression of Wx proteins, allergen-like proteins and elongation factor 1 β,
but increased expression of small heat shock proteins (sHSP),
glyceraldehyde-3-phosphate dehydrogenase and prolamin. sHSP was positively
correlated with the appearance of chalky kernels. In this study, we used RT-PCR to
confirm the expression profiles of those genes.
Subjects
rice
proteomics
high temperature
grain quality
Wx protein
heat shock
protein
protein
水稻
蛋白質體學
高溫
稻米品質
Waxy 蛋白質
熱休克蛋白
Publisher
臺北市：國立臺灣大學農藝學系暨研究所
Type
report
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