Comparative Proteomics Analysis of Salmonella enterica serovar Choleraesuis Isolates
Date Issued
2007
Date
2007
Author(s)
Chen, Chih-Kuan
DOI
zh-TW
Abstract
Salmonella Choleraesuis is one of the most important pathogens for pigs. It occasionally infects human and causes serious systemic infection. Although antibiotics are the effective drug to prevent and cure the disease, evidences suggest that more and more isolates of S. Choleraesuis in Taiwan have developed drug resistance, especially to flouroquinolone (FQ), and the cases of human infection are increasing. In the last few years, studies have been devoted to genotyping of S. Choleraesuis for epidemiology, but only few have been made at the expression patterns of genes or proteins of S. Choleraesuis. In this study, the proteomics analysis was performed to determine the differential patterns of proteins. Four different isolates were chosen from 199 isolates isolated from Taiwan by Centers for Disease Control: one FQ resistant isolate and one FQ susceptible isolate from human, and one FQ resistant isolate and one FQ susceptible isolate from pig. After the differential displayed proteins were identified by 2D-electrophoresis, the expression level of individual protein was confirmed by Real Time QPCR. Finally, fourteen candidate proteins were identified. Of which, seven proteins could be associated with FQ resistance: SC064, sat, dps, groS, groL, pduB, malE, six proteins could be related to host specificity: sat, dps, groL, pduB, menB, STY3221, and five proteins could be related to FQ resistance in different hosts：sat, dps, groL, pduB, menB, STY3221. The results suggested that S. Choleraesuis could possess universal mechanisms in adaptation to FQ and host by regulating the expression of dps and HSPs. S. Choleraesuis might suffer more stress within human than in pigs, as the genes related to growth and development were down-regulated. S. Choleraesuis might have different drug resistant within different hosts via isolated evolution, therefore human isolates appear to have higher drug resistance.
Subjects
霍亂沙門氏桿菌
抗藥性
寄主性
二維電泳
即時偵測定量聚合脢連鎖反應
獨立演化
Salmonella Choleraesuis
Drug resistance
Host specificity
Real Time QPCR
Isolate evolution
SDGs
Type
other
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