Proteomic Analysis of Protein Expression Profiles during Early Developmental Stages of Zebrafish, Danio rerio
Date Issued
2008
Date
2008
Author(s)
Yang, Ya-Ting
Abstract
All processes that occur before the activation of the zygotic genome at midblustula transition are driven by maternally inherited products, which have been synthesized and stored in mature oocytes during oogenesis. After fertilization, these maternal factors initiate developmental cascades that carry out the embryonic developmental program. To investigate the dynamic expressed pattern of these maternal factors, total proteomic analysis was performed during two transitions, oocyte-to-embryo transition (OET) and maternal-to-zygotic transition (MZT), in zebrafish early development. Using 1D SDS-PAGE and LC-MS/MS, we identified 1109 proteins and categorized them based on their biological functions. Many proteins involved in translation or post-translational modification were actively expressed during the selected five stages. This result implied that maternal mRNAs required for subsequent developmental processes were translated rapidly under well control after fertilization. Some of the proteins with differential expression during OET or MZT are proteins involved in cell-cycle regulation, development processes, and zygotic genome activation. The dynamic expressions of these proteins assisted embryos in progressing these two important transitions and subsequent developmental program. A comparison of our proteomic results with transcriptomic data obtained in other studies revealed that some of the proteins are maternally deposited since no transcripts can be detected in these selected stages. In other words, these protein levels did not correspond to their mRNAs amounts during these stages. This result confirmed idea that proteomics or transcriptomics approach alone cannot reveal the complete picture about any biological process; instead, both approaches are complementary and essential for the description of biological events at molecular levels. In this study, we provide a new resource for the selection of candidate proteins for targeted quantitation and refined systematic genetic network analysis in vertebrate development in biology.
Subjects
Zebrafish early embryonic development
Maternal factor
oocyte-to-embryo transition (OET)
maternal-to-zygotic transition (MZT)
LC-MS/MS
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