In Vivo and In Vitro Molecular Imaging by Using Third Harmonic Generation Microscopy
Date Issued
2007
Date
2007
Author(s)
Tai, Shih-Peng
DOI
en-US
Abstract
In this thesis, we demonstrated the first optical harmonics generation microscope (HGM) in the world by collecting backward propagating second harmonic generation (SHG) and third harmonic generation (THG) signals. We also applied the developed epi-HGM on the study of dermatology and oral oncology. The signals of optical harmonics generation is generated from endogenous structures in materials and biological specimens without stain. HGM has no energy deposition in the specimens due to the energy conservation in optical harmonics generation process. Therefore, HGM is one of least invasive optical microscopy technique. SHG and THG are quadratic and cubic dependent on incident laser power and thus efficient optical harmonics generation can be observed only near the focal point. By using a high NA objective, the spatial resolution of HGM can achieve sub-
Subjects
三倍頻
雷射掃瞄顯微術
影像對比劑
量子點
奈米粒子
Third harmonic generation
laser scanning microscopy
contrast agents
quantum dots
nanoparticles
SDGs
Type
thesis
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