Epidemiological Investigation of Bovine Leptospirosis from Beef Abattoir and Dairy Farms
Date Issued
2004
Date
2004
Author(s)
Lin, Nian-Nong
DOI
zh-TW
Abstract
To understand the status of bovine leptospirosis in Taiwan, we collected 480 serum samples and corresponding kidney tissues from one abattoir near Taoyuan and 570 serum samples from 15 dairy farms in Hualien and Taitung counties, during the period between August, 2003 and April, 2004. The leptospiral antibody was examined by microscopic agglutination test (MAT). A titer ≧1:100 was considered as positive. The polymerase chain reaction (PCR) and bacteria isolation using EMJH medium were employed for detection of Leptospira bacteria in kidney tissues. The kidneys fixed in 10% formalin were routinely prepared for the sections of H&E and Warthin-Starry staining and immunohistochemically (IHC) stained with LipL32 antibody for detection of Leptospira. Twenty seven point five percent (132/480) of bovine sera from abattoir were positive by MAT. One hundred and eight samples were positive to one, and 24 samples were positive to more than one, which had titres between 1:100 and 1:3200 with geometric mean titer equal to 1:175. The most common serogroup is Pomona. One of four hundred eighty kidney sample was PCR positive, which belongs to genomospecies of L. interrogans after an initial examination. One of the seventy eight kidney samples which have white spot was IHC positive. The results of Warthin-Starry staining and culture are all negative. McNemar test There are significant association between both macroscopic renal lesions, multi-focal interstitial nephritis (MFIN) and anti-leptospiral antibody (p<0.01) by McNemar test. The study in Hualien and Taitung counties, we evaluated the serology and risk factors in 19 dairy farms. The positive rate of all herds were 100%. Total positive rate was 37.8% (263/695), which had titres between 1:100 and 1:400 with geometric mean titer equal to 1:131. The most serogroup was Shermani. Main risk factor associated with seropositive was presence of animals around the farm (OR=1.7; p<0.01). This serological results probably reflected natural exposure because vaccination of cattle against leptospirosis was not practiced in Taiwan. These results represent economic loss and potential threats to human health. From the investigation, bovine leptospirosis is prevalent in Taiwan. A successful control program for bovine leptospirosis should enforce a comprehensive investigation on the infection and the herd management practices.
Subjects
牛鉤端螺旋體症
小白點
危險因子
多發局部間質性腎炎
bovine leptospirosis
white spot
multi-focal inte
SDGs
Type
thesis
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