Modulation of the Proliferation and Function of Skeletal Myoblast and ligament on peptide-conjugated Submicro-grooved Surfaces
Date Issued
2011
Date
2011
Author(s)
Wu, Tsung-Han
Abstract
Cell-substrate interaction is a key point for tissue engineering, and many studies have focused on mimetic of the cell’s natural tissue environment to investigate the cellular behaviors. Both skeletal muscles and ligaments are constructed in bundles of fibers in human body, and the requirements for cell-guidance in aligned shape in vitro can be successful reached by the submicro-grooved pattern substrates. There are two approaches for cell behavior observation which are chemical signal, such as extracellular matrix (ECM) signals; and physical signals, such as topography effect, structural of substrates, and surrounding fluids. In this study, both of these two signals were designed to investigate the effect to cell proliferation and functionalities. The RGD- and YIGSR- containing PLGA patterned surfaces not only proved to enhance the proliferation and differentiation of cell but they also improved the focal adhesion and cytoskeleton presentation. The dopamine-coated and RGD-conjugated pattern surfaces also showed an enhancement of cellular proliferation and secretion of ECM content for ligament fibroblast, which was observed by cell number detection and fluorescent staining. The results indicated that both chemical signals from ECM-derivates and physical signals that mimetic the nature alignment shape of cell can successfully enhance cell proliferation and functionalities in vitro.
Subjects
submicron
grooved patterm
peptide
skeletal muscle
ligament
Type
thesis
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