Phage Display in Selecting Peptides to Functionalize Titanium Alloy
Date Issued
2009
Date
2009
Author(s)
Siaw, Yang-Khim
Abstract
Since Prof. Branemark introduced the methodology of osseointegrated dental implantology in 1960s, the development of dental implantation technology has become mature and the success rate of treatment has reached an incredible level of more than 90 percent in average. Titanium is the most widely used metal in orthopedic joint replacement, fixation screws and plates, and dental implants. Titanium is preferred over other metal materials because it provides good biocompatibility and corrosion resistance with very low allergenicity. Even though titanium owns such superiority, some problems and limitations of its application in dental implantation still remain to be improved. For instance, when titanium dental implants are used, patients are generally obliged to endure restricted mastication for several weeks until the tight adhesion and osseointegration between the implant and local tissues is established. In addition, dentists also face the failure of implantation and other complications, such as progressive marginal bone loss or recession of marginal mucosa, in spite of its high success rate reported in the literature. Therefore, shortening the time required for osseointegration and enhancing the success rate of implantation through appropriate implant modification are major objectives to substantially improve patients’ life quality. To achieve those objectives, a variety of methods for surface modification of titanium implants have been proposed, including plasma flame spraying, sandblasting, acid-etching, and coating with ceramic. In this study, we screened a phage displayed random peptide library for searching peptides that can specifically bind onto titanium surface and then we identified the binding properties of those titanium binding peptides. We identified two phage clones named TBP-A and TBP-E showing different binding affinities after the biopanning procedures. The corresponding peptides were then synthesized and studied with the following competitive binding inhibition with original phage clone and synthetic peptide binding experiments. Among all the synthetic peptides, TBP-E2 has dominant binding affinity compared to others The novel peptide that can specifically bind to titanium may be good a candidate to immobilize growth factors onto implant surface to achieve the goal of improving titanium surface.
Subjects
Phage Display
Surface Treatment
Titamium Implant
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