Optimization of somatic embryogenesis and plantlet regeneration of celery (Apium graveolens L.) via suspension cell culture
Date Issued
2004
Date
2004
Author(s)
Liao, Pei-Yu
DOI
zh-TW
Abstract
植物體胚發生(somatic embryogenesis)為利用植物細胞所具之全潛能性(totipotency)，以體細胞誘導不定胚之技術。體胚細胞培養技術可應用於植物育種(交雜育種、轉殖)以及開發人工種子。本研究以芹菜(Apium graveolens L.)葉為培植體, 利用SH培養基(含2,4-D 7.5
Due to totipotency of plant cells, somatic cells of plants inherently tend to produce vegetative propagation through somatic embryogenesis. The investigation of somatic embryogenesis motivates an agricultural breeding and a large-scale artificial seed system. The celery (Apium graveolens L.) was used as a model system to investigate the induction and cultivation conditions of embryo cells. At first, leaves were used to induce the callus (SH basal medium, 2,4-D 7.5
Subjects
人工種子
芹菜
密度梯度離心
體胚發生
植株再生
density gradient centrifugation
celery
artificial seed
somatic embryogenesis
plantlet regeneration
Type
thesis
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