The Relation of Environment Exposure and Allelic Loss in Esophageal Cancer
Date Issued
2002-07-31
Date
2002-07-31
Author(s)
李章銘
DOI
902314B002431
Abstract
Esophageal cancer is still an important disease in Taiwan. Various environmental exposures have
been associated with the risk of esophageal cancer. In the previous study, we have found that the
risk of esophageal cancer can be significantly increased by the habits of cigarette smoking, alcohol
drinking and areca chewing. The risk was positively correlated with the exposure dosage of these
factors. These three factors also act synergistically to enhance the individual susceptibility of
esophageal cancer. In the recent project, using the technique of laser capture microdissection
(LCM) and polymerase-chain-reaction-based polymorphic markers located on chromosomes 17 and
18, we have detected multiple allelic deletions (LOH) on these areas. Some of the LOHs are
compatible with the locations of the known tumor suppressor genes(TSG), i.e. p53 [17p13.1],
NF1[17q11.2], BRCA1[17q21], SMAD4[18q21], DCC [18q21.3]. However, some of them are
not, indicating more potential tumor suppressor genes are yet to be found in these areas. We
found that the areca chewers had a significantly higher tendency for retention of heterozygocity in
17p12 (p<0.05, as compared to non-chewers). It implies that the environmental exposure may
determine the mutation or expression in specific tumor suppressor genes due to the different
mechanisms of the chemicals-associated carcinogenesis. To further clarify this, we proposed the
study to investigate the association of p53 mutation and environmental exposure of the patients.
We used the technique of LCM to obtain the tumor genomic DNA. P53 sequencing was
3
performed after PCR amplification using primers locating on exons 4-9 of p53 gene. At the
present time, we have finish the work of sequencing on exons 4, 6 and 9 of p53. Form the
preliminary data, we found that mutation pattern is affected by the environmental exposure of the
patients.
Subjects
Esophageal cancer
p53
mutation
SDGs
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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