A wound-healing assay based on ultraviolet light ablation
Journal
SLAS Technology
Journal Volume
22
Journal Volume
22
Journal Issue
1
Journal Issue
1
Pages
36-43
Start Page
36
End Page
43
ISSN
24726303
Date Issued
2017-02
Author(s)
Abstract
Collective cell migration plays important roles in many physiological processes such as embryonic development, tissue repair, and angiogenesis. A “wound” occurs when epithelial cells are lost and/or damaged due to some external factors, and collective cell migration takes place in the following wound-healing process. To study this cellular behavior, various kinds of wound-healing assays are developed. In these assays, a “wound,” or a “cell-free region,” is created in a cell monolayer mechanically, chemically, optically, or electrically. These assays are useful tools in studying the effects of certain physical or chemical stimuli on the wound-healing process. Most of these methods have disadvantages such as creating wounds of different sizes or shapes, yielding batch-to-batch variation, and damaging the coating of the cell culture surface. In this study, we used ultraviolet (UV) lights to selectively kill cells and create a wound out of a cell monolayer. A comparison between the current assay and the traditional scratch assay was made, indicating that these two methods resulted in similar woundhealing rates. The advantages of this UV-created wound-healing assay include fast and easy procedure, high throughput, and no direct contact to cells.
Subjects
Collective cell migration
Ultraviolet light
Wound-healing assay
SDGs
Publisher
SAGE Publications Inc.
Type
journal article