Characterization of lead nanoparticles formed by Shewanella sp. KR-12
Journal
Journal of Nanoparticle Research
Journal Volume
18
Journal Volume
18
Journal Issue
1
Journal Issue
1
Pages
1-10
Start Page
1
End Page
10
ISSN
13880764
Date Issued
2016-01-01
Author(s)
Liu, Chien-Liang
Abstract
The bacterial strain KR-12 was isolated from river sediment in northeast Taiwan. 16S rRNA gene sequencing revealed that it belongs to the genus Shewanella. The strain can accumulate lead (Pb) and form Pb nanoparticles (PbNPs) on exposure to Pb(NO3)2 and sodium formate in HEPES buffer. On transmission electron microscopy (TEM), the KR-12-formed PbNPs were spherical in shape and ranged from 3 to 8 nm. The PbNPs formed a line or curved pattern on bacteria. In addition, one or more pilus-like structures elongated from the bacteria. In contrast, Shewanella oneidensis MR-1 and other bacteria could not form PbNPs pattern or pilus-like structure under the same conditions. High-resolution TEM combined with energy-dispersive X-ray spectroscopy demonstrated that these PbNPs primarily contained Pb and had an amorphous structure. This is the first report of the biosynthesis of PbNPs by a Shewanella species.
Subjects
Bacteria
Biosynthesis
Microbiology
Pb Nanoparticles (PbNPs)
Pilus-like structure
Shewanella
SDGs
Publisher
Springer Netherlands
Type
journal article