Optimization of Glucose Release from Taro Agricultural Resource for Lactobacillus acidophilus Cultivation and Evaluation of Its Biological Activity Properties
Date Issued
2014
Date
2014
Author(s)
Pua, Xiao-Hui
Abstract
Food manufacturing sectors, using starch crop, fruits and vegetables as input, has generated a huge amount of field waste and processing wastes that can be utilized as agricultural resources. Yet, the current applications of taro agricultural resources (TAR) was limited as fertilizers. On the other hand, recent studies have revealed that non-viable probiotics have many therapeutic activities including anti-tumor and immunoregulatory properties. However, studies on the influence of culturing medium towards the biological activities of non-viable probiotics were scarce. In this study, TAR was utilized to produce glucose as carbon source for Lactobacillus acidophilus BCRC 14079 cultivation and the anti-tumor and immunomudulatory property of non-viable probiotics namely heat-killed cell (HKC), cytoplasmic fraction (CF) and exopolysaccharide (EPS) were evaluated. Optimum parameter for liquefaction determined by Box-Behnken Design Response Surface Methodology (BBD-RSM) was 79.2oC of temperature, 9 mL/L of α-amylase enzyme solution and 5 h of reaction time, producing 30.57 g/L of reducing sugar. For saccharification, dose of amyloglucosidase, temperature, and saccharification time were determined as 0.3mL/L of amyloglucosidase enzyme solution, 60oC and 3 h, resulting in approximately 60.14 g/L of glucose. Various alternative nitrogen sources, namely bone meal (BM), fish meal (FM), chicken meal (CM), dried distillers grains with solubles (DDGS), corn gluten meal (CGM), and soy meal (SM) were evaluated to substitute yeast extract (YE) as nitrogen source in order to produce a more economic and environmental friendly medium for lactic acid bacteria (LAB) cultivation. The most potential novel TAR medium for enhanced LAB growth, CGMY1 medium, constitutes of approximately 37 g/L of glucose (TAR hydrolyzate) and CGM supplemented with 1 g/L of YE, producing log 9.20 CFU/mL of LAB cells. MTT assay result showed HKC and EPS from CGM medium exhibited the highest anti-proliferative effect towards colon adenocarcinoma cells HT-29 and Caco-2. In addition, the anti-tumor effect of HKC was better than EPS. The results of luciferase-based NF-ΚB and COX-2 system indicated that HKC from CGM medium stimulates the most expression of luciferin reporter in both systems, followed by EPS from CGMY1 medium and CF from MRS medium. In conclusion, this study demonstrated the potential of utilizing TAR for L. acidophilus cultivation and the production of non-viable probiotics with enhanced anti-tumor and/or immunoregulatory property by using novel TAR mediums.
Subjects
芋頭農業資材
嗜酸乳酸菌
熱致死菌
胞外多醣
免疫調節
抗腫瘤
SDGs
Type
thesis
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