水腦對感覺運動皮質的影響：細胞結構之研究
Date Issued
2005-10-31
Date
2005-10-31
Author(s)
郭夢菲
DOI
932314B002242
Abstract
Hydrocephalus, a pathological enlargement of the cerebral ventricles resulted from
oversecretion, abnormality of flow, or malabsorption of cerebrospinal fluid is known to cause
motor, behavioral and cognitive deficits in children and adults. Animal and human studies thus far
indicate damages to structures along the periventricular area, in particular nerve fibers of the
white matter. However, despite the dramatic thinning of the cortex above the lateral ventricle, no
studies have dedicated to understand the cytoarchitecture and individual neuron status of the
affected cortical area. In this study, we use Nissl and anti-PGP9.5, GAD, and NOS as neuron
markers and the lectin GSA I-B4 and anti-GFAP, CD11b, ED-1 as glial markers to study the
changes of the cytoarchitecture of the affected cortex, the sensorimotor cortex. In addition, we
use fixed-tissue intracellular dye injection technique to study the soma-dendritic arbors of the
layer III and V pyramidal neurons, the cortical output neurons, of the affected sensorimotor
cortex. The technique and its subsequent immunoconversion and reconstruction reveals the
complete soma-dendritic morphology of neurons selected for injection and allows detailed
analysis of the extent of their dendritic arbors, branching profuseness, dendritic length and
dendritic spine densities. These detailed morphological data can reveal the functional status of
these neurons under compression. In addition, we performed neuronal tracing in live slices in a
custom-designed incubation chamber to reveal the short-distance projections of these two types
of pyramidal neurons to find out whether they still control their targets. These data are crucial to
determine how the compressed cortex may function during hydrocephalus.
Subjects
Hydrocephalus
astrocytes
cerebral cortex
intracellular dye injection
Lucifer yellow
microglia
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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