Design and Development of Handheld Surface Plasmon Resonance Meter
Date Issued
2006
Date
2006
Author(s)
Chen, Yi-Hao
DOI
zh-TW
Abstract
Surface plasmon resonance, SPR, is a charge-density oscillation that exists at the interface of two media with dielectric constants of opposite signs. When the momentum of incident light equals to the momentum of surface plasmon wave, surface plasmon resonance will be excited. The general ways of surface plasmon resonance modulation are angle modulation, wavelength modulation, phase modulation, and intensity modulation. Owing to the advantages of simple, rapid, and high sensitivity, intensity modulatuin meets the requirements of hand-held surface plasmon resonance meter. Surface plasmon resonance is sensitive to the thin film region that is about 150nm above the metal surface. The light inten-sity reflected by the metal responses to the changes of optical parameters of the thin film. In measuring bio-molecule interactions, surface plasmon resonance has the advantages of non-labeling, real-time, and small sample volume, so the sur-face plasmon resonance meter has become the leading technology in bio-sensing application. We view bio-molecules layer as an optical film, the changes of re-flective index produced by binding reactions and density changes are plotted to record the bio-molecules characteristic curve.
The opto-mechanism volume has reduced from the table size to the hand-held size in this dissertation. Instead of using laser as light source, a 2 inch back-light module combined with parabolic mirror introduces parallel light to carry out milti-channels experiments. The whole dimemsions of handheld surface plasmon resonance meter are 4 x 6 x 1.5 cm3. To surpress the environmental noise, envi-ronmental light, a sealed mounting set was applied to allow no ambient light en-tering. Moreover, to improve signal to noise ratio, the reference channel was added in the optical path to cancel out the light source and temperature distur-bance. Handheld surface plasmon resonance meter is applied to antibody-antigen assay. After completing alcoholic test, the bio-molecules binding test, human IgG and anti-IgG, were than completed qualitatively. The obtained reflective intensity described the kinetic analysis and proved effectiveness of this newly develope.
Subjects
表面電將共振
手持式
光強調制
折射率
SPR handheld intensity modulation reefractive index
Type
thesis
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