Development of Saturation Pulse Fluorescence Imaging System and Its Applications to Plants under Light-Heat Stress
Date Issued
2006
Date
2006
Author(s)
Hsieh, Lu-Lin
DOI
zh-TW
Abstract
In this article, the saturation pulse fluorescence imaging system (SPFIS) was developed to analyze to physical status of plants under light and heat stress. One part of SPFIS, the light plane system, was controlled by a programmable logic controller (PLC) to supply three different lighting conditions, including weak modulated measuring beam, continuous actinic light and saturating pulse lights, during measuring period.
The other part of SPFIS, fluorescence imaging system, was developed to grab images with different signal gain setting of the CCD camera according to the different lighting conditions. The function of gain setting was used to overcome the insufficiency of camera dynamic range. Besides, the imaging frequency of SPFIS was auto adjusted to increase the time resolution when the fluorescence reaction change quickly and to decrease the sampling rate when the fluorescence reaction is stable. The imaging time was also recorded. The serial fluorescence images and the time record of plants were computed by the designed image processes to extract the complete fluorescence image information.
The plants, under normal and different phases of light and heat stress, were analyzed by SPFIS to obtain three fluorescence results, including non-photochemical quenching of chlorophyll fluorescence, non-photochemical quenching curve of chlorophyll fluorescence, and intensity image of fluorescence index. Compared with the measurement results of the same plant by mini-PAM, SPFIS had the ability to detect the variation of physical status when plants were under stress.
Moreover, according to SPFIS could obtain the complete fluorescence reaction of full leaves and consider the variations of full leaves under stress, the measurement results showed more consistent than mini-PAM, when plants were under stress condition. Saturation pulse fluorescence imaging system provides another way for research workers to monitoring plant physiological in non-destroy style.
Subjects
螢光影像
植物逆境
飽和脈衝法
光合作用
Fluorescence imaging
Plant stress
Saturation Pulse Method
Photosynthesis
Type
thesis
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