Detection of urine cofilin-1 from patients hospitalized in the intensive care unit using the metal-enhanced fluorescence technique
Journal
Sensors and Actuators, B: Chemical
Journal Volume
173
Date Issued
2012-10-01
Author(s)
Chang, Ying Feng
Chen, Hsien Ching
Lin, Lih Yuan
Yu, Pei Chun
Chang, Yu Sun
Lee, Yi Jang
Chou, Chien
Abstract
Ischemic shock and acute kidney injury (AKI) are the primary causes of high mortality in the intensive care unit (ICU) patients. In a rat model, ischemia is able to disrupt the actin cytoskeleton of proximal tubule cells. This effect is associated with the expression and excretion of actin depolymerizing factor (ADF)/cofilin. However, the human evidence of ADF/cofilin excreted in ICU patients with ischemic shock and/or AKI remains to be addressed. Here we developed a 96-well high-throughput, localized surface plasmon-coupled fluorescence biosensor (HT-LSPCFB) combining a sandwich immunoassay to measure the urine cofilin-1 in 57 ICU patients and 8 healthy controls. A linear relationship between 1 and 10 5 pg/mL of human cofilin-1 recombinant protein was determined (R 2 = 0.9845) in this study. The highest normalized cofilin-1 levels obtained in the ICU patients and healthy adults were 1.985 and 0.726, respectively. The mean normalized cofilin-1 level of total ICU patients (0.7403) was also significantly higher than that of healthy adults (0.4759) (p = 0.0052). An examination of the receiver operating characteristic (ROC) curve and area under curve (AUC) showed that cofilin-1 is acceptable for assessing the ICU patients (AUC = 0.775) and shock (AUC = 0.713), but not for AKI (AUC = 0.681). Therefore, the HT-LSPCFB is suitable for detecting the urine cofilin-1 which potentially becomes a prognostic factor for ICU patients with shock. © 2012 Elsevier B.V.
Subjects
Cofilin-1 | HT-LSPCFB | Intensive care unit | Ischemic shock and acute kidney injury
SDGs
Type
journal article