Analysis of the Steps Involved in Dengue Virus Entry into Host Cells
Resource
VIROLOGY v.257 n.1 pp.156-167
Journal
VIROLOGY
Journal Volume
v.257
Journal Issue
n.1
Pages
156-167
Date Issued
1999
Date
1999
Author(s)
HUNG, SHAN-LING
CHEN, LI-KUANG
KAO, CHUAN-LIANG
KING, CHWAN-CHUEN
Abstract
The initial steps of dengue viral entry have been divided into adsorption and penetration using acid glycine treatment to inactivate extracellular virus after attachment to baby hamster kidney (BHK) cells but prior to penetration. First, we showed that virus infection was accomplished within 2 h after adsorption. Second, the assay was used to examine the properties of dengue envelope E protein-specific monoclonal antibodies ( MAbs), lectins, and heparin. We found that three MAbs, 17-2, 46-9, and 51- 3, may neutralize dengue 2 virus ( DEN-2) through inhibition of not only viral attachment but also of penetration. However, one MAb, 56-3.1, interfered specifically with attachment. Therefore, the functional domains of E protein involved in attachment and penetration may be different. Moreover, studies with lectins indicated that carbohydrates, especially alpha-mannose residues, present on the virion glycoproteins may contribute to binding and penetration of the virus into BHK and mosquito C 6/36 cells. Finally, virus
Subjects
Dengue
Virus Entry
VIrus Attachment
Pathogenesis
Monoclonal Antibody
Mosquito
SDGs
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