Effect of folate status on high fat diet experimental autoimmune encephalomyelitis mice and T cell activity
Date Issued
2015
Date
2015
Author(s)
LEE, Chien-Wei
Abstract
Multiple sclerosis (MS) is the prototypical inflammatory demyelinating disease of the central nervous system (CNS). Experimental Autoimmune Encephalomyelitis (EAE), which is a common animal model of Multiple sclerosis, is mediated by myelin specific CD4+ helper T cells. Th1 as well as Th17 phenotype is the major T helper cells in EAE pathogenesis. It is considered that regulatory T cell plays a protective role in pathogenic process. Recent studies showed that folate deficiency may enhance the inflammatory response of macrophages. Besides, folate can also reduces the activiton and proliferation of immune cell. In this study, we used EL4 T lymphocyte to investigate the effects of folate status on immune activation in vivo, and an animal model was used to investigate the effects of folate status on EAE-induced mice, which were fed with high fat diet. The EL4 T cell line was cultured in different folate status medium. The data showed that folate deficiency significantly increased IL-2 secretion and its mRNA expression. Moreover, the IL-2 transcription factor NFAT5 and NFκB were upregulated. The results showed that folate deficiency may promote higher IL-2 secretion via activating NFκB and NFAT5 pathway. The male C57BL6/J mice were divided into four groups fed an AIN-96G high fat diet (54% of total calorie) which containing either 0 (Hf0), 2 (Hf1), 20 (Hf10) mg folate per kg diet. Anthor group was fed AIN-96G hight fat diet only for 3 weeks before EAE induced (NF/HF).After 10 weeks, use MOG35-55 to induced EAE mode, and sacrifice at 15 day after EAE induced.The data showed that the C57BL/6 male mice, which were fed folate-deficient high fat diet or short-period high fat diet, had serious peripheral and brain inflammation. There were less Th1, Th17, and regulatory T cells that infiltrated into CNS of these mice. However, there is no sign of serious clinical symptoms on EAE. In comparison, the mice fed with folate-supplement high fat diet had more serious clinical symptoms on EAE and brain inflammation. Besides, there were more regulatory T cells infiltrated into CNS.
Subjects
Multiple sclerosis
Experimental Autoimmune Encephalomyelitis
Folate
Regulatory T cell.
Type
thesis
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