Study on the relatedness of LsGRP1 to the salicylic acid-induced resistance of Lilium cv. Star Gazer by agroinfiltration
Date Issued
2006
Date
2006
Author(s)
Peng, Hsien-Chieh
DOI
zh-TW
Abstract
Lilium is a bulbous crop, severely affected by a major fungal disease, gray mold, caused by Botrytis elliptica. As know, development of necrotic lesions in the leaves of Oriental lily cultivar Star Gazer could be suppressed by the application of salicylic acid (SA). A cDNA sequence, named LsGRP1, coding for a 138-amino acid protein has been identified via suppression subtractive hybridization and differential screening. When the lily plants were treated with SA solution, the amount of LsGRP1 transcript analyzed by Northern blot hybridization increased. In the study of the relatedness between LsGRP1 and salicylic acid-induced disease resistance, agroinfiltration was applied for analysis of LsGRP1 transient expression in the leaves of Lilium cv. Star Gazer. In Northern blot hybridization, LsGRP1 transcript increased 72 hours after agroinfiltration of sense strand LsGRP1–expressing Agrobacterium tumefaciens EHA101 strain and more obviously 96 hours after agroinfiltraion. When the lily plants were treated with SA solution, the amount of LsGRP1 mRNA decreased in ‘Star Gazer’ leaves infiltrated with A. tumefaciens EHA101 strains expressing sense, anti-sense and hairpin LsGRP1, but the lesion numbers increased as compared to that in the control. These results showed that a gene silencing reaction was induced by introducing sense, anti-sense or hairpin LsGRP1, that abated the resistance of ‘Star Gazer’ leaves to gray mold disease. Thus, LsGRP1 playing an important role in salicylic acid-induced disease resistance of lily wasconcluded.
Subjects
葵百合
LsGRP1
基因靜默
短暫表現
Lilium cv. Star Gazer
agroinfiltration
gene silencing
transient expression
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-95-R92633010-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):b3727f80cfd10fc471a240ec14d44b52