Toll-like receptor 3 expression inhibits cell invasion and migration and predicts a favorable prognosis in neuroblastoma
Journal
Cancer Letters
Journal Volume
336
Journal Issue
2
Pages
338-346
Date Issued
2013
Author(s)
Abstract
To evaluate the clinical significance of TLR3 expression on neuroblastomas, we performed immunohistochemical study on archival tissues and in vitro studies on neuroblastoma cell lines. The results showed that positive TLR3 expression was associated with favorable histology and prognosis. Activation of TLR3 by polyinosinic:polycytidylic acid [poly(I:C)] treatment is effective to suppress cell migration and invasion and to decrease organized assembly of F-actin and filopodia formation, in TLR3-expressing SK-N-AS cells, which could be reversed by TLR3-targeting siRNA treatment. TLR3 agonist poly(I:C) promotes GAP-43 expression also in SK-N-AS cells only. Taken together, TLR3 could serve to predict favorable behavior in neuroblastomas. ? 2013 Elsevier Ireland Ltd.
SDGs
Other Subjects
F actin; messenger RNA; neuromodulin; polyinosinic polycytidylic acid; small interfering RNA; toll like receptor 3; article; cancer prognosis; cell invasion; cell migration; child; controlled study; female; filopodium; gene expression; human; human cell; human tissue; immunohistochemistry; in vitro study; infant; major clinical study; male; neuroblastoma; neuroblastoma cell; preschool child; priority journal; protein expression; school child; Actins; Apoptosis; Cell Line, Tumor; Cell Movement; Child; Child, Preschool; Female; GAP-43 Protein; Gene Expression; Humans; Infant; Infant, Newborn; Male; Neuroblastoma; Poly I-C; Prognosis; RNA, Small Interfering; Signal Transduction; Toll-Like Receptor 3
Type
journal article