Feasibility study of using montmorillonite to improve the stability of L-Ascorbic acid
Date Issued
2007
Date
2007
Author(s)
Lin, Kun-Yu
DOI
zh-TW
Abstract
As L-ascorbic acid (LAA) is unstable in nature. This study is trying to use montmorillonite to provide feasible strategy to stabilize LAA for practical uses. In addition, using acid treated montmorillonite (MMT) to compare the effect of stabilizing LAA that formulate by low toxic phosphoric acid. To analyze the property changed of modified MMT by XRD (X-ray diffraction)、EDS（Energy Dispersive X-ray Spectrometer）、BET analyzer. According to Single-beam scanning UV/VIS spectrophotometer showed that the decomposition rate of LAA was decreased by adding MMT (In 12 hours, the LAA (0.001%w/v) delayed 97% of activity; In contract, LAA mixed with MMT (0.1%w/v) will only decompose 28%.). In addition, adding less amount of modified MMT (0.01 N acid treated) than MMT could had the same effect of stabilizing LAA.
Toxicity assessment also showed that MMT treated with low level acids should be safe according to our assessment. The test of dermal fibroblast proliferation showed that the 10 %w/v LAA could assist fibroblast proliferation (The optical density value was increased 11 fold). The experiment of dermal fibroblast proliferation also appeared that adding MMT or acid treated MMT would not had harmful effect on dermal fibroblast. Thus, using MMT or acid treated MMT to stabilize LAA might be a feasible method of LAA for practical uses.
Subjects
蒙脫石
左旋維他命C
酵母菌
人體皮膚纖維母細胞
montmorillonite
l-ascorbic acid
Saccharomyces cerevisiae
human dermal fibroblast
Type
thesis
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