Three-dimensional refractive-index microscope for analyzing red blood cells
Date Issued
2015
Date
2015
Author(s)
Chen, Ching-An
Abstract
Anemia is a common disease caused by low amount of red blood cells (RBCs)/hemoglobin in the blood, or a lowered ability of the blood to carry oxygen. The symptoms include fatigue, dyspnea, confusion or syncope. Volume and hemoglobin contents of the RBCs are the indices to determine if the patient has the anemia symptom in the initial blood test. The current method in the clinical hospital is the fully-automated whole blood analyzer for calculating the indices of RBCs. Using this method, the RBCs analysis can be finished in a short time and acquire high throughput data. The fully-automated whole blood analyzer is good for RBCs clinical analysis in the initial blood test although it also needs more manual process by clinical laboratory technologist when the machine detects the abnormal state on the blood. Furthermore, the automated machine cannot provide morphology of the RBCs. The goal of the thesis is to establish a method that can reconstruct 3D morphology of RBCs and quantify the volume and hemoglobin contents by a novel optical microscopy technique: three-dimensional (3D) refractive-index (RI) microscopy which is based on digital holography and optical diffraction tomography. Using this technique, we can reconstruct the 3D-RI mapping of the BRCs and evaluate the deviation between individual RBCs without staining or other extra process. 3D-RI microscopy have been used for the measurement of normal and thalassemia RBCs and the statistical result of volume and Hb concentration are compared with the clinical blood tests. The 3D-RI microscopy was practically performed on mapping 3D-RI distribution of RBCs and we found that the RI and morphology difference between normal and the thalassemia patients are clear to be observed in our results. In the future, we will measure more RBCs from normal and anemia patients with different symptoms to establish a more reliable statistics.
Subjects
Red
blood
cells
imaging
hemoglobin
anemia
holography
three-dimensional refractive index image
Type
thesis
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