Antiviral activity of Vigna radiata extract against feline coronavirus in vitro
Journal
Veterinary Quarterly
Journal Volume
44
Journal Issue
1
Start Page
1
End Page
13
ISSN
01652176
Date Issued
2024-01-01
Author(s)
Chou, Ai-Ai
Lin, Chung-Hui
Lin, Yi-Chen
Pi, Chia-Chen
Kan, Yao-Ming
Chuang, Hao-Fen
DOI
10.1080/01652176.2024.2349665
Abstract
Feline infectious peritonitis (FIP) is a fatal illness caused by a mutated feline coronavirus (FCoV). This disease is characterized by its complexity, resulting from systemic infection, antibody-dependent enhancement (ADE), and challenges in accessing effective therapeutics. Extract derived from (L.) R. Wilczek (VRE) exhibits various pharmacological effects, including antiviral activity. This study aimed to investigate the antiviral potential of VRE against FCoV, addressing the urgent need to advance the treatment of FIP. We explored the anti-FCoV activity, antiviral mechanism, and combinational application of VRE by means of antiviral assays. Our findings reveal that VRE effectively inhibited the cytopathic effect induced by FCoV, reduced viral proliferation, and downregulated spike protein expression. Moreover, VRE blocked FCoV in the early and late infection stages and was effective under ADE infection. Notably, when combined with VRE, the polymerase inhibitor GS-441524 or protease inhibitor GC376 suppressed FCoV more effectively than monotherapy. In conclusion, this study characterizes the antiviral property of VRE against FCoV , and VRE possesses therapeutic potential for FCoV treatment.
Subjects
Feline coronavirus
GC376
GS-441524
Vigna radiata extract
antiviral
feline infectious peritonitis
Type
journal article