A Reusable Biosensor Using Glutathione-Modified Silicon Nanowire Field-Effect Transistor
Date Issued
2009
Date
2009
Author(s)
Tseng, Kun-Chang
Abstract
Over past years, the silicon nanowire field-effect transistors (SiNW-FETs) as chemical and biological sensors have attracted wide attention because of their merits of free labeling, ultrasensitivity, and real-time recording. There are many applications of SiNW-FETs, such as immuno-protein detection, DNA hybridization, virus detection, etc. However, most of applications are based on an irreversible functionalization of receptor molecules on the surface of SiNW-FETs. Consequently, one chip that could only be used for a single time to detect one protein is very wasteful in chip-consuming. lutathione-S-transferase (GST) is widely applied to fuse with a target protein by genetic engineering in protein purification. The GST-fused protein can be separated from a lysate by the glutathione-coated beads via its high affinity for glutathione (GSH).ased on the reversible association-dissociation of GSH-GST, we provide a novel strategy of using a reusable GSH modified SiNW-FET to immobilize a GST-fusion protein and then to screen possible interacting proteins. The reversible surface-functionalization method is proven by ESCA, fluorescence imaging, atomic force microscopic imaging, and electrical measurements. The reversible GSH-GST functionality on the SiNW-FET has made this device consecutively reusable, allowing for quantitative analysis. In this work, we have successfully demonstrated that the reusability of GSH-functionalized device should be a very useful and economic biosensor. his reversible surface functionalized biosensor can potentially serve as a fast high-throughput screening platform for detecting proteins which could possibly interact with a particular GST-fused protein, and also be further applied to study biomolecular associations, such as protein-protein interactions, protein-DNA interactions, protein-carbohydrate interactions, and other similar interactions.
Subjects
silicon nanowire
field-effect transistor
reusable
glutathione
biosensor
Type
thesis
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