Studies of Free-solution Electrophoresis of Model ssDNA by Monte Carlo Simulations
Date Issued
2005
Date
2005
Author(s)
Chen, Yan-Ren
DOI
zh-TW
Abstract
It is well-known that uniformly charged polyelectrolytes such as DNA migrate with the same mobility regardless of chain length. However, they may be separated by free solution electrophoresis if an uncharged particle is attached to the one end of the polyelectrolyte. On the basis of Monte Carlo simulations, the mobility μ of a polyelectrolyte of length Nc attached with an neutral polymer of length Nu is investigated. According to the Rouse (free-draining) picture, it is anticipated that the mobility becomes size-dependent and follows μ(Nc) ∝ Nc/(Nc+Nu). The additional resistance provided by the neutral section breaks the balance between the electric driving force (Nc) and hydrodynamic drag (Nc+ Nu). Our simulation results indicate that although the end label offers the broken symmetry but the aforementioned relation is not valid due to the interaction friction. With both ends are labeled with neutral polymers of Nu* and Nu-Nu*, it is found that the mobility increases as Nu*/(Nu-Nu*)→1. In weak electric field, the conformation may change from random coil to charge-neutral segregation regime as the ratio Nc/(Nc+ Nu) is decreased. Because the conformations are different with/without electric field, the Nernst-Einstein equation is not valid for end-labeled polyelectrolytes.
Subjects
堆疊
開啟與密合
蒙地卡羅法
環狀結構
自由溶液電泳
尾端
stack
ELFSE
drag tag
Monte Carlo simulations
end-labeled
open-to-closed transition
size-independent
Type
thesis
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