Extracts from fermented black soybean milk exhibit antioxidant and cytotoxic activities
Journal
Food Technology and Biotechnology
Journal Volume
49
Journal Issue
1
Pages
111-117
Date Issued
2011
Author(s)
Abstract
In this study, ethanol extracts from 2-day fermented black soybean milk (FBE) by immobilized Rhizopus oligosporus NTU5 have been evaluated for both antioxidant and cytotoxic activities. The results reveal that a 2-day FBE had strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging effect (76 %). The extracts were further fractionated by silica gel column chromatography and an unknown compound, FBE5-A, was obtained, which exhibited strong antioxidant activity. IC50 of the DPPH scavenging effect of FBE5-A was 7.5 £gg/mL, which is stronger than a commonly used antioxidant, vitamin E (£\-tocopherol; 17.4 £gg/mL), and similar to vitamin C (ascorbic acid; 7.6 £gg/mL). The cytotoxic test demonstrated that extracts of 2-day fermented broth exhibited selective cytotoxic activity towards human carcinoma cells, Hep 3B (IC50=150.2 £gg/mL), and did not affect normal human lung fibroblasts, MRC-5 (p<0.05). The results indicate the potential applications of fermented black soybean milk as functional food, pharmaceutical or cancer therapy formula.
Subjects
Antioxidant activity
Black soybean
Cytotoxic activity
Isoflavone
Rhizopus oligosporous
SDGs
Other Subjects
Glycine max; Rhizopus; Rhizopus microsporus var. oligosporus
Type
journal article