Protective effect of Cordyceps militaris extract on myocardial injury and osteoporosis in chemotherapy mice
Journal
Nutritional Sciences Journal
Journal Volume
46
Journal Issue
2
Date Issued
2022-01-01
Author(s)
Abstract
Chemotherapy in cancer patients is prone to cause cardiotoxicity and osteoporosis. In the past, it has been reported that has anti-tumor, immune regulation and other health benefits. Whether has the effect of alleviating adverse side effects caused by chemotherapy remains to be explored. Therefore, this study mainly explored whether the addition of extract had cardioprotective and osteoporotic effects on H9c2 cardiomyocytes and ICR female mice treated with adriamycin chemotherapy. In this experiment, DPPH detection was used to show that the extract of extract had excellent antioxidant capacity. MTT assay was used to show that the extract has an excellent protective effect on damaged myocardial cell by adriamycin. Enzyme-linked immunosorbent assay (ELISA) was used to show that the extract can enhance IgG and IgA expressions, but inhibit IgE expression in the serum of chemotherapy mice. Immunohistochemical staining and Western blotting methods was used to show that the extract can enhance antioxidant-related SOD2 protein, but inhibited inflammatory-related TNF- protein expressions in myocardial tissue of chemotherapy mice. Computer tomography was used to show that the effect of increasing the bone density of tibia tissue of chemotherapy mice. Based on the above re-extract has the sults, the extract should have protective and relieving effects on cardiotoxicity and osteoporosis induced by chemotherapy in mice.
Subjects
cancer | cardiotoxicity | chemotherapy | Cordyceps militaris | inflammation | osteoporosis | oxidative stress
SDGs
Type
journal article