The physiological response and protein expression profile of different rice (Oryza sativa L.) cultivars under high temperature stress
Date Issued
2004
Date
2004
Author(s)
Lao, Chia-Ling
DOI
zh-TW
Abstract
In Taiwan, high temperature causes the reduction of tiller number and spikelet sterility in rice plants thus affects the final yield and grain quality. Our objective is to determine the physiological response and protein expression profile of different rice cultivars under high temperature stress. Ours data showed that after 4hr of 45℃ heat treatment than recovered at 30/25℃ for 2 days caused rice seedlings decrease in chlorophyll content, chlorophyll fluorescence, and a marketed increase in leakage, MDA content. The indica rice tends to be more thermotolerant than japonica rice according to the change of morphological symptoms and physiological assay. Among 22 rice cultivars, a high temperature sensitive cultivar（Tainung 67, TNG67）and high temperature tolerant cultivar（Taichung Sen 10, TCS10）were selected for further use in study of heat stress tolerance by SDS-PAGE and 2-D electrophoresis. The result showed that either japonica or indica rice, a 60kDa protein was induced in leaf but not in root tissue under high temperature treatment. The western blot analysis also showed high temperature tolerant cultivar（TCS10）expressed more LMW sHSPs under heat stress. With 2-D gel analysis, several proteins which up-regulated by high temperature in ether TCS10 or TNG67 were identified, including：sHSPs, SEC protein, wall-associated protein kinase, peroxin6 and ACC synthase. The function of these proteins may be correlated with thermotolerance by protein phosphorylation、HSP accumulation、protein membrane transport、ethylene biosynthesis. Interesting enough, one of the protein, mannose-binding rice lectin（MRC）is down-regulated by heat stress in TCS10 and expressed in low level in TNG67 either with or without high temperature treatment. After protein database search, this cultivar-specific expressed protein shows highly similarity to salT gene which is induced by salt. The function of this protein may correlate to thermotolerance of rice.
Subjects
蛋白質體學
生理
水稻
high temperature stress
rice
physiological response
protein expression profile
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-93-R91621101-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):885da769775f76a353f65865a727056b