In vivo multiphoton imaging and quantification of cytoplasmic and nuclear metabolism in the hepatobiliary system
Journal
Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Journal Volume
10069
Date Issued
2017
Author(s)
Abstract
Liver performs xenobiotic excretion out of hepatocytes with metabolic function. However, hepatocellular metabolism was non-uniform in hepatocyte. Hepatocellular metabolism could be different in nucleus and cytoplasm. In this study, we use the molecular probe 6-carboxyfluorescein diacetate (6-CFDA) to simulate xenobiotic metabolism in hepatocytes with multi-photon fluorescence microscopy in vivo. 6-CFDA was processed by intracellular esterase to 6- carboxyfluorescein (6-CF) with green fluorescence. And this probe was used to study differences in cytoplasmic and nuclear metabolism of hepatocytes.
SDGs
Publisher
SPIE
Type
conference paper