MicroRNA-519c Suppresses Hypoxia-Inducible Factor-1{alpha} Expression and Tumor Angiogenesis
Resource
CANCER RESEARCH, 70(7), 2675-2685
Journal
CANCER RESEARCH
Journal Volume
70
Journal Issue
7
Pages
2675-2685
Date Issued
2010-04
Date
2010-04
Author(s)
CHA, SHIH-TING
CHEN, PAI-SHENG
CHU, CHIA-YU
WANG, MING-YANG
JENG, YUNG-MING
YU, SUNG-LIANG
CHEN, JIN-SHING
CHANG, KING-JEN
JEE, SHIOU-HWA
TAN, CHING-TING
LIN, MING-TSAN
KUO, MIN-LIANG
Abstract
Hypoxia-inducible factor-1 alpha (HIF-1 alpha) is widely considered to be one of the key regulators of tumor angiogenesis . The upstream regulation is complex and involves several growth factors, cytokines, and hypoxia. Herein, we have identified miR-519c as a hypoxia-independent regulator of HIF-1 alpha, acting through direct binding to the HIF-1 alpha 3' untranslated region and leading to reduced tumor angiogenesis . Overexpression of miR-519c resulted in a significant decrease of HIF-1 alpha protein levels and reduced the tube formation of human umbilical vein endothelial cells; similarly, antagomir inhibition of miR-519c increased the level of HIF-1 alpha protein and enhanced angiogenic activity, suggesting an important role of miR-519c in HIF-1 alpha-mediated angiogenesis. Consistent with the overexpression of miR-519c in cancer patients with better prognosis, mice injected with miR-519c- overexpressing cells exhibited dramatically reduced HIF-1 alpha levels, followed by suppressed tumor angiogenesis , growth, and metastasis. In addition, we found that hepatocyte growth factor (HGF), a known HIF-1 alpha inducer, reduced the miR-519c levels through an Akt-dependent pathway. This regulation was post-transcriptional and may be mediated by suppression of miR-519c maturation. Taken together, our findings provide the first evidence that miR- 519c is a pivotal regulator of tumor angiogenesis and that microenvironmental HGF contributes to regulating miR-519c biogenesis in cancer cells.
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