Mechanism of biofilm formation and antibiotics resistance in pathogenic E. coli O157
Date Issued
2005
Date
2005
Author(s)
Liu, Hsua-Hon
DOI
en-US
Abstract
In previous studies, we found that adding some antibiotics to E. coli DH5a induced its biofilm formation. The 2-D gel electrophoresis showed that some proteins were expressed more and some less upon the treatment. In the present study, bacitracin and kanamycin were used to treat the pathogenic E. coli O157. We first improved the technology of running 2-D gel and clearly identified the proteins with different expression level. The proteins including TnaA, PspA, MenB, TnaA, Aco2, EFG, and PspA were expressed more in the cytoplasm of E. coli O157 upon incubation with bacitracin. Proteins CH10, EftS, TalB, UidR, PhojH, PtnA, and AroB were expressed more upon kanamycin treatment. Among them, TnaA (tryptophanase) catalyzes the formation of indole from Tryptophane and indole can secret into the medium to reach a critical concentration (250 uM), thereby triggering the biofilm formation. Its regulatory mechanism was then investigated.
Besides indole, a small quantity of an unknown autoinducer was released to the medium while the E. coli O157 was treated with bacitracin. In the present study, we treated the mutants of transposon gene knockout with the partially purified autoinducer to examine the response of the mutant bacteria. We discovered B3355, YdgR, YaaA, BglG, and B0359 that could not form biofilm. This allowed the identification of important proteins involved in the autoinducer signaling and biofilm formation, which may serve as anti-biofilm drug targets.
Subjects
病原菌大腸桿菌
生物膜
蛋白質體學
pathogenic E. coli
biofilm
proteomics
Type
other
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